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Chapter 3: Classification of Bacteria

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Eubacteria that lack cell walls and do not synthesize the precursors of peptidoglycan are called

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(A) Gram-negative bacteria

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(B) Viruses

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(C) Mycoplasmas

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(D) Serovar variant

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(E) Bacilli

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Archaebacteria can be distinguished from eubacteria by their lack of

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(A) DNA

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(B) RNA

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(C) Ribosomes

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(D) Peptidoglycan

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(E) Nucleus

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A 16-year-old cystic fibrosis patient is admitted to the hospital. A sputum culture yields Burkholderia cepacia. Subsequently, there are two other patients with B cepacia bacteremia, and the organism is cultured from the sputum of four additional patients. During this nosocomial outbreak of B cepacia, 50 environmental and 7 patient isolates are being subtyped to identify the source of the outbreak. Which of the following techniques would be most useful in this endeavor?

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(A) Culture

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(B) Ribotyping

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(C) 16S rRNA sequencing

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(D) Antimicrobial susceptibility testing

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(E) Nucleic acid sequencing

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An unculturable gram-positive microorganism has been visualized in tissue specimens obtained from patients with a previously undescribed disease. Which of the following techniques would be most useful in identifying this organism?

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(A) Serology

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(B) PCR amplification and sequencing of rRNA genes

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(C) Multilocus enzyme electrophoresis

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(D) SDS-polyacrylamide gel electrophoresis

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(E) Pulsed field gel electrophoresis

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The DNA polymerase from Thermus aquaticus is an important component of DNA amplification methods such as the polymerase chain reaction. This organism is capable of growing at temperatures above 100°C. Organisms that are capable of growth at these temperatures are referred to as

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(A) Mesophiles

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(B) Psychrophiles

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(C) Halophiles

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(D) Thermophiles

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(E) Chemolithotrophs

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A bacteria with a genome having a G + C content of 45% harbors a plasmid encoding a gene with a G + C content of 55%. Which ...

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