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Chapter 5: Cultivation of Microorganisms

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Most microorganisms pathogenic for humans grow best in the laboratory when cultures are incubated at

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(A) 15–20°C

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(B) 20–30°C

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(C) 30–37°C

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(D) 38–50°C

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(E) 50–55°C

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The process by which microorganisms form ATP during the fermentation of glucose is characterized by

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(A) Coupling of ATP production with the transfer of electrons

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(B) Denitrification

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(C) The reduction of oxygen

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(D) Substrate phosphorylation

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(E) Anaerobic respiration

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The major effect of a temperature of 60oC on the growth of a mesophile such as Escherichia coli is to

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(A) Destroy the cell wall

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(B) Denature proteins

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(C) Destroy nucleic acids

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(D) Solubilize the cytoplasmic membrane

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(E) Cause formation of endospores

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Polymerization of building blocks (eg, amino acids) into macromolecules (eg, proteins) is achieved largely by

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(A) Dehydration

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(B) Reduction

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(C) Oxidation

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(D) Assimilation

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(E) Hydrolysis

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A strain of E coli does not require vitamins when grown in a defined medium consisting of glucose, mineral salts, and ammonium chloride. This is because E coli

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(A) Does not use vitamins for growth

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(B) Obtains vitamins from its human host

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(C) Is a chemoheterotroph

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(D) Can synthesize vitamins from the simple compounds provided in the medium

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(E) Ammonium chloride and mineral salts contain trace amounts of vitamins

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Which of the following is NOT a mechanism for generating metabolic energy by microorganisms?

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(A) Fermentation

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(B) Protein synthesis

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(C) Respiration

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(D) Photosynthesis

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(E) C and D

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Which of ...

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