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Chapter 7: Microbial Genetics

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Mutations in bacteria can occur by which of the following mechanisms?

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(A) Base substitutions

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(B) Deletions

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(C) Insertions

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(D) Rearrangements

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(E) All of the above

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The form of genetic exchange in which donor DNA is introduced to the recipient by a bacterial virus is

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(A) Transformation

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(B) Conjugation

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(C) Transfection

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(D) Transduction

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(E) Horizontal transfer

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The enzyme DNAse degrades naked DNA. If two strains of bacteria from the same species were mixed in the presence of DNAse, which method of gene transfer would be most likely inhibited?

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(A) Conjugation

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(B) Transduction

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(C) Transformation

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(D) Transposition

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(E) All of the above

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Replication of which of the following requires physical integration with a bacterial replicon?

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(A) Single-stranded DNA bacteriophage

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(B) Double-stranded DNA bacteriophage

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(C) Single-stranded RNA bacteriophage

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(D) Plasmid

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(E) Transposon

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The formation of a mating pair during the process of conjugation in Escherichia coli requires

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(A) Lysis of the donor

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(B) A sex pilus

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(C) Transfer of both strands of DNA

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(D) A restriction endonuclease

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(E) Integration of a transposon

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Why do bacteria contain restriction enzymes?

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(A) To cleave RNA for incorporation into ribosome

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(B) To extend the length of bacterial chromosomes

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(C) To prevent foreign DNA from incorporating into a bacterial genome

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(D) To process the exons from prokaryotic mRNA

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(E) To proteolytically cleave nuclear promoters

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If the arrangement of bases on the coding DNA strand is 5′CATTAG3′, ...

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