TY - CHAP M1 - Book, Section TI - Enzymes: Mechanism of Action A1 - Kennelly, Peter J. A1 - Rodwell, Victor W. A2 - Rodwell, Victor W. A2 - Bender, David A. A2 - Botham, Kathleen M. A2 - Kennelly, Peter J. A2 - Weil, P. Anthony Y1 - 2018 N1 - T2 - Harper's Illustrated Biochemistry, 31e AB - OBJECTIVESAfter studying this chapter, you should be able to:Describe the structural relationships between specific B vitamins and certain coenzymes.Outline the four principal catalytic mechanisms and how they can be combined by enzymes to facilitate chemical reactions.Explain the concept of an “induced fit” and how it facilitates catalysis.Outline the underlying principles of enzyme-linked immunoassays.Describe how coupling an enzyme to the activity of a dehydrogenase can simplify many enzyme assays.Identify proteins whose plasma levels are used for diagnosis and prognosis.Describe the application of restriction endonucleases and of restriction fragment length polymorphisms in the detection of genetic diseases.Illustrate the utility of site-directed mutagenesis for the identification of aminoacyl residues that are involved in the recognition of substrates or allosteric effectors, or in the mechanism of catalysis.Describe how “affinity tags” can facilitate purification of a protein expressed from its cloned gene.Indicate the function of specific proteases in the purification of affinity-tagged enzymes.Discuss the events that led to the discovery that RNAs can act as enzymes, and briefly describe the evolutionary concept of an “RNA world.” SN - PB - McGraw-Hill Education CY - New York, NY Y2 - 2024/03/28 UR - accesspharmacy.mhmedical.com/content.aspx?aid=1160188986 ER -